pBMN-Z
(Plasmid #1734)

• Purpose: This is Nolan lab's standard retrovirus vector backbone that expresses lacZ
• Depositing Lab: Garry Nolan
• Publication: Nolan Lab Plasmids (unpublished)

Backbone

• Vector backbone: pBMN
• Backbone manufacturer: Nolan Lab
• Backbone size w/o insert (bp): 8396
• Vector type: Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: LacZ

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pBMN 5'

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is Nolan lab's standard retrovirus vector backbone that expresses lacZ. Most of the other library vectors and IRES constructs are based on this expression vector. A variant comes with GFP in place of the lacZ gene (see pBMN-I-GFP).

Please note that there are some minor differences between Addgene's quality control sequence (see sequence with the pLXSN-5 primer) and the assembled sequence from the Nolan lab. See the following reference for further information: digitalcommons.unl.edu/cgi/viewcontent.cgi?article=1008&context=chemistrycerny