LgBiT-hACE2
(Plasmid
#173431)
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Purposeprotein expression plasmid of LgBiT-hACE2-IgG1 Fc
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 173431 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonephCMV3
- Backbone size w/o insert (bp) 4200
- Total vector size (bp) 7300
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Vector typeMammalian Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLgBiT-hACE2-IgG1 Fc
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Alt namelarge bit of split luciferase, fused with human ACE2, human IgG1 Fc region
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SpeciesH. sapiens (human)
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Insert Size (bp)3100
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GenBank ID59272
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Entrez GeneACE2 (a.k.a. ACEH)
- Promoter CMV
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Tag
/ Fusion Protein
- IgG1 (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40 pA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LgBiT-hACE2 was a gift from David Nemazee (Addgene plasmid # 173431 ; http://n2t.net/addgene:173431 ; RRID:Addgene_173431) -
For your References section:
A Rapid Assay for SARS-CoV-2 Neutralizing Antibodies That Is Insensitive to Antiretroviral Drugs. Huang D, Tran JT, Peng L, Yang L, Suhandynata RT, Hoffman MA, Zhao F, Song G, He WT, Limbo O, Callaghan S, Landais E, Andrabi R, Sok D, Jardine JG, Burton DR, Voss JE, Fitzgerald RL, Nemazee D. J Immunol. 2021 Jul 1;207(1):344-351. doi: 10.4049/jimmunol.2100155. Epub 2021 Jun 28. 10.4049/jimmunol.2100155 PubMed 34183368
Map uploaded by the depositor.
