BPK1520_puroR
(Plasmid
#173901)
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Purpose(Empty Backbone) SpCas9 guide cloning vector derived from BPK1520 that allows for puromycin-based selection
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 173901 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneBPK1520
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Backbone manufacturerJoung Lab (Addgene Plasmid #65777)
- Backbone size (bp) 3636
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Modifications to backboneCMV-driven puromycin resistance cassette
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Vector typeMammalian Expression, CRISPR
- Promoter CMV/U6
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byPuromycin resistance cassette was cloned from PX459 V2.0 pSpCas9 (BB)-2A-Puro (Addgene Plasmid #62988). CMV promoter was cloned from pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA (Addgene Plasmid #61591)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
BPK1520_puroR was a gift from Ronald Cohn (Addgene plasmid # 173901 ; http://n2t.net/addgene:173901 ; RRID:Addgene_173901) -
For your References section:
Modeling Niemann-Pick disease type C in a human haploid cell line allows for patient variant characterization and clinical interpretation. Erwood S, Brewer RA, Bily TMI, Maino E, Zhou L, Cohn RD, Ivakine EA. Genome Res. 2019 Dec;29(12):2010-2019. doi: 10.1101/gr.250720.119. Epub 2019 Nov 21. 10.1101/gr.250720.119 PubMed 31754021
