|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17418||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2574
Vector typeBacterial Expression, Yeast Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameGALL promoter + polylinker + CYC1 terminator
Alt nametruncated GAL1 (long) promoter
SpeciesS. cerevisiae (budding yeast)
Insert Size (bp)2353
MutationGALL promoter is between SacI and XbaI restriction sites.
Entrez GeneGAL1 (a.k.a. YBR020W)
- Cloning method Restriction Enzyme
- 5′ cloning site BglI (not destroyed)
- 3′ cloning site BglI (not destroyed)
- 5′ sequencing primer M13 Reverse (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byPlasmid was created by Paula Calle. Insert was cut from Mumberg plasmid p425GALL, which was obtained from ATCC.
Terms and Licenses
Mumberg D, Muller R, Funk M. Nucleic Acids Res. 25:5767-8 (1994).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p404GALL was a gift from Nicolas Buchler & Fred Cross (Addgene plasmid # 17418)