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p404MET25
(Plasmid #17420)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 17420 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    p404ADH1
  • Backbone size w/o insert (bp) 2581
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MET25 promoter + polylinker + CYC1 terminator
  • Alt name
    MET17 promoter (official name in SGD)
  • Alt name
    MET15 promoter
  • Alt name
    O-acetyl homoserine-O-acetyl serine sulfhydrylase
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2301
  • Mutation
    MET25 promoter is between SacI and XbaI restriction sites.
  • Entrez Gene
    MET17 (a.k.a. YLR303W, MET15, MET25)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglI (not destroyed)
  • 3′ cloning site BglI (not destroyed)
  • 5′ sequencing primer M13 Reverse
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Plasmid was created by Paula Calle. Insert was cut from Mumberg plasmid p425MET25, which was obtained from ATCC.
  • Terms and Licenses

Depositor Comments

Mumberg D, Muller R, Funk M. Nucleic Acids Res. 25:5767-8 (1994).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p404MET25 was a gift from Nicolas Buchler & Fred Cross (Addgene plasmid # 17420)