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p406GALL
(Plasmid #17434)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 17434 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    p406ADH1
  • Backbone size w/o insert (bp) 2691
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GALL promoter + polylinker + CYC1 terminator
  • Alt name
    truncated GAL1 (long) promoter
  • Alt name
    Galactokinase
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2353
  • Mutation
    GALL promoter is between SacI and XbaI restriction sites.
  • Entrez Gene
    GAL1 (a.k.a. YBR020W)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglI (not destroyed)
  • 3′ cloning site BglI (not destroyed)
  • 5′ sequencing primer M13 Reverse
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Plasmid was created by Paula Calle. Insert was cut from Mumberg plasmid p425GALL, which was obtained from ATCC.
  • Terms and Licenses

Depositor Comments

Mumberg D, Muller R, Funk M. Nucleic Acids Res. 25:5767-8 (1994).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p406GALL was a gift from Nicolas Buchler & Fred Cross (Addgene plasmid # 17434)