Modified Shipping Schedule: Addgene will be closed November 23rd & 24th for the Thanksgiving holiday. Order processing and shipping may be delayed the week of Nov 20 - 24. If you have any questions, please contact us at [email protected].
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

p406MET3
(Plasmid #17436)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 17436 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    p406MET3-cyc1t
  • Backbone manufacturer
    A. Geoghegan (Rockefeller University)
  • Backbone size w/o insert (bp) 4844
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MET3 promoter + polylinker + CYC1 terminator
  • Alt name
    ATP sulfurylase
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    260
  • Mutation
    MET3 promoter is between SacI and XbaI restriction sites.
  • Entrez Gene
    MET3

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer M13 Reverse
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Original vector p406MET3-cyc1t was created by subcloning PCR fragment of genomic MET3 promoter (with SacI and XbaI restriction sites introduced) into SacI-XbaI cut vector pRS406. This plasmid does not contain CYC1 terminator. Plasmid p406MET3 was created by subcloning SalI-KpnI insert from p405MET25 (insert = CYC1 terminator) into SalI-KpnI cut p406MET3-cyc1t vector.
  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p406MET3 was a gift from Nicolas Buchler & Fred Cross (Addgene plasmid # 17436)