Addgene - MSCV-FOXP3-IRES-Thy1.1 Plasmid Data

Plasmid 17443: MSCV-FOXP3-IRES-Thy1.1

Gene/insert name:
FOXP3
Species:
H. sapiens (human)
Entrez Gene:
FOXP3 (JM2, AIID, DIETER, IPEX, PIDX, XPID)
Vector backbone:
MSCV-IRES-Thy1.1 DEST
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
6063
Cloning method:
Gateway Cloning
Cloning site 5':
attR1
Site destroyed during cloning:
Yes
Cloning site 3':
attR2
Site destroyed during cloning:
Yes
5' sequencing primer:
na List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers, other:
Thy1.1
Sequence: View sequences (1)
Principal Investigator:
Anjana Rao
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: FOXP3 controls regulatory T cell function through cooperation with NFAT. Wu et al (Cell. 2006 Jul 28. 126(2):375-87. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17443" in your Materials and Methods section.