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pET23 P53DBD
(Plasmid #174503)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 174503 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET23a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3666
  • Total vector size (bp) 4269
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    P53 DNA binding domain (corresponds to amino acid 94-312)
  • Alt name
    TP53
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    662
  • Entrez Gene
    TP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)
  • Promoter T7
  • Tag / Fusion Protein
    • none

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 Terminator
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET23 P53DBD was a gift from Stewart Loh (Addgene plasmid # 174503 ; http://n2t.net/addgene:174503 ; RRID:Addgene_174503)
  • For your References section:

    Structure, function, and aggregation of the zinc-free form of the p53 DNA binding domain. Butler JS, Loh SN. Biochemistry. 2003 Mar 4;42(8):2396-403. doi: 10.1021/bi026635n. 10.1021/bi026635n PubMed 12600206