Addgene - pENTR/pTER shLUC (w177-1) Plasmid Data

Plasmid 17472: pENTR/pTER shLUC (w177-1)

Gene/insert name:
Firefly Luciferase shRNA
Alt name:
shLUC
Insert size:
65
Vector backbone:
pENTR1A
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
RNAi ; Entry vector
Backbone size w/o insert (bp):
2556
Cloning site 5':
Bgl II
Site destroyed during cloning:
Yes
Cloning site 3':
Hind III
Site destroyed during cloning:
No
5' sequencing primer:
ENTRforw List of Sequencing Primers
3' sequencing primer:
ENTRrev
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
TOP10F', 37oC
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Eric Campeau
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Comments:

The pENTR/pTER plasmid was derived from the pTER plasmid (van de Wetering et al. 2003)

shRNA oligo sequence is 5'-CCTTACGCTGAGTACTTCGAGTGTGCTGTCCTCGAAGTACTCAGCGTAAGTTT

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau et al (PLoS One. 2009 Aug 6;4(8):e6529. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17472" in your Materials and Methods section.