Addgene - pQCXP CMV/TO LUC (w432-1) Plasmid Data

Plasmid 17476: pQCXP CMV/TO LUC (w432-1)

Gene/insert name:
Firefly Luciferase
Insert size:
1652
Vector backbone:
pQCXP
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral, Luciferase
Backbone size w/o insert (bp):
7189
5' sequencing primer:
CMVforw List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
TOP10F', 37oC
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Eric Campeau
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Comments:

Luciferase gene derived from the pGL3 plasmid (Stratagene)

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau et al (PLoS One. 2009 Aug 6;4(8):e6529. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17476" in your Materials and Methods section.