Addgene - pBRIT Pax7 216C Plasmid Data

Plasmid 17523: pBRIT Pax7 216C

Gene/insert name:
Pax7
Insert size:
850
Species:
M. musculus (mouse)
Entrez Gene:
Pax7 (RP23-334I5.3, Pax-7)
Fusion protein or tag:
FLAG
Terminal:
C terminal on backbone
Mutation:
Deletion of aa 1-215
Vector backbone:
pBRIT-LoxP-HA/FLAG-puro
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral, Cre/Lox
Backbone size w/o insert (bp):
5200
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
pBABE5' List of Sequencing Primers
3' sequencing primer:
pBABE3'
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Michael Rudnicki
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Pax7 activates myogenic genes by recruitment of a histone methyltransferase complex. McKinnell et al (Nat Cell Biol. 2008 Jan . 10(1):77-84. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17523" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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