pBRIT Pax7 N33-170C
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17526||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5200
Vector typeMammalian Expression, Retroviral, Cre/Lox
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Insert Size (bp)1100
MutationDeletion of aa 34-159 (paired domain)
Entrez GenePax7 (a.k.a. Pax-7)
/ Fusion Protein
- FLAG (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer pBABE5'
- 3′ sequencing primer pBABE3' (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBRIT Pax7 N33-170C was a gift from Michael Rudnicki (Addgene plasmid # 17526)
For your References section:Pax7 activates myogenic genes by recruitment of a histone methyltransferase complex. McKinnell IW, Ishibashi J, Le Grand F, Punch VG, Addicks GC, Greenblatt JF, Dilworth FJ, Rudnicki MA. Nat Cell Biol. 2008 Jan . 10(1):77-84. 10.1038/ncb1671 PubMed 18066051
Map uploaded by the depositor.