Addgene - CG4845/pMT/V5-HisB Plasmid Data

Plasmid 17589: CG4845/pMT/V5-HisB

Gene/insert name:
CG4845
Insert size:
3000
Species:
D. melanogaster (fly)
GenBank ID:
NM_142601
Entrez Gene:
psidin (Dmel_CG4845, CG4845, Dmel\CG4845, ird16)
Fusion protein or tag:
6x His
Terminal:
C terminal on backbone
Fusion protein or tag:
V5
Terminal:
C terminal on backbone
Vector backbone:
pMT/V5-His B
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Insect Expression
Backbone size w/o insert (bp):
3500
Cloning site 5':
Kpn1
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
MT-Fwd List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Denise Montell
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17589" in your Materials and Methods section.