Addgene - pRK5-HA-Ubiquitin-K63 Plasmid Data

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Plasmid 17606: pRK5-HA-Ubiquitin-K63

Gene/insert name:
Ubiquitin C
Alt name:
Ub
Insert size:
220
Species:
H. sapiens (human)
Entrez Gene:
UBC (HMG20)
Fusion protein or tag:
HA
Terminal:
N terminal on backbone
Mutation:
K63 only, other lysines mutated to arginines
Vector backbone:
pRK5-HA
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert:
4800
Cloning site 5':
SalI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
SP6 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Ted Dawson
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Parkin mediates nonclassical, proteasomal-independent ubiquitination of synphilin-1: implications for Lewy body formation. Lim et al (J Neurosci. 2005 Feb 23. 25(8):2002-9. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17606" in your Materials and Methods section.