Addgene - LRRK2-WT Plasmid Data

Plasmid 17609: LRRK2-WT

Gene/insert name:
LRRK2
Insert size:
7380
Species:
H. sapiens (human)
Entrez Gene:
LRRK2 (AURA17, DARDARIN, PARK8, RIPK7, ROCO2)
Fusion protein or tag:
Myc
Terminal:
C terminal on backbone
Fusion protein or tag:
His
Terminal:
C terminal on backbone
Vector backbone:
pcDNA3.1 myc/his A
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5500
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
XhoI
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
3' sequencing primer:
BGH-rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
30
Growth instructions:
Stbl3, 30 degrees
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (5)
Map:
View map
Principal Investigator:
Ted Dawson
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Parkinson's disease-associated mutations in leucine-rich repeat kinase 2 augment kinase activity. West et al (Proc Natl Acad Sci U S A. 2005 Nov 15;102(46):16842-7. Epub 2005 Nov 3. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17609" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

Print

This is commonly requested with

17613: pRK5-HA-Parkin

25045: pDEST53-LRRK2-G2019S

17608: pRK5-HA-Ubiquitin-WT

17612: pRK5-Myc-Parkin

25366: 2XMyc-LRRK2-3XKD