Addgene - pRK5-HA-Parkin Plasmid Data

Plasmid 17613: pRK5-HA-Parkin

Gene/insert name:
Parkin
Insert size:
1400
Species:
H. sapiens (human)
Entrez Gene:
PARK2 (KB-152G3.1, AR-JP, LPRS2, PDJ, PRKN)
Fusion protein or tag:
HA
Terminal:
N terminal on backbone
Vector backbone:
pRK5-HA
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4800
Cloning site 5':
SalI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
SP6 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Ted Dawson
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Parkin functions as an E2-dependent ubiquitin- protein ligase and promotes the degradation of the synaptic vesicle-associated protein, CDCrel-1. Zhang et al (Proc Natl Acad Sci U S A 2000 Nov 21;97(24):13354-9. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17613" in your Materials and Methods section.