|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17616||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backboneSIN LV pRLLhPGK.GFP Sin-18 (modified)
Backbone manufacturerDr. Didier Trono
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Gene/Insert nameEF-1a promoter
Alt nameEF-1 alpha promoter
SpeciesH. sapiens (human)
Insert Size (bp)1200
/ Fusion Protein
- GFP (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer EGFP-N (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made bySIN LV pRLLhPGK.GFP Sin-18 (abbreviated as PGK.GFP) was kindly provided by Dr. Didier Trono.
Terms and Licenses
Articles Citing this Plasmid
Addgene sequencing results have found a 10 bp insert between nt 6201 and 6202 of the depositor's full plasmid sequence and a C6203G mutation. These differences are not believed to affect the function of the plasmid.
SIN lentiviral vector that expresses GFP controlled by the promoter of the housekeeping gene EF-1alpha.
We constructed EF.GFP by replacing the human PGK promoter (EcoRV to BamHI sites in the PGK.GFP vector) with human EF-1alpha promoter from plasmid pEF1/Myc-His-A (Invitrogen, Carlsbad, CA). The EF-1alpha promoter (nucleotide 373 to 1560 as numbered in GenBank accession J04616) contains the first exon, first intron, and part of the second exon before the coding sequence (starting at nucleotide 1582).
Recombinant LVs were generated using the 3-plasmid system by cotransfection of 293T cells through calcium phosphate precipitation. The ratio of a transducing vector, pMD.G, and pCMVDeltaR8.91 was fixed at 1.5:0.5:2 µg for 10^6 293T cells.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:EF.GFP was a gift from Linzhao Cheng (Addgene plasmid # 17616)
For your References section:Targeting transgene expression to antigen-presenting cells derived from lentivirus-transduced engrafting human hematopoietic stem/progenitor cells. Cui Y, Golob J, Kelleher E, Ye Z, Pardoll D, Cheng L. Blood. 2002 Jan 15. 99(2):399-408. 10.1182/blood.V99.2.399 PubMed 11781219