|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17617||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backboneSIN LV pRLLhPGK.GFP Sin-18 (modified)
Backbone manufacturerDr. Didier Trono
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Gene/Insert nameHLA-DRa promoter
Alt name(MHC II) HLA-DR alpha promoter
SpeciesH. sapiens (human)
Insert Size (bp)300
Entrez GeneHLA-DRA (a.k.a. HLA-DRA1)
/ Fusion Protein
- GFP (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer EGFP-N (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made bySIN LV pRLLhPGK.GFP Sin-18 (abbreviated as PGK.GFP) was kindly provided by Dr. Didier Trono.
Terms and Licenses
SIN lentiviral vector that expresses GFP controlled by the promoter of the human HLA-DRalpha gene, which is selectively expressed in antigen-presenting cells (APCs).
We constructed DR.GFP by using the human (MHC II) HLA-DRalpha promoter (nucleotide 182 to 480, as numbered in GenBank accession X00274) to replace the PGK promoter (XhoI to BamHI sites in the PGK.GFP vector). This 300-base pair promoter sequence has been shown to confer selective expression of a transgene in MHC II+ cells.
Recombinant LVs were generated using the 3-plasmid system by cotransfection of 293T cells through calcium phosphate precipitation. The ratio of a transducing vector, pMD.G, and pCMVDeltaR8.91 was fixed at 1.5:0.5:2 µg for 10^6 293T cells.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:DR.GFP was a gift from Linzhao Cheng (Addgene plasmid # 17617)
For your References section:Targeting transgene expression to antigen-presenting cells derived from lentivirus-transduced engrafting human hematopoietic stem/progenitor cells. Cui Y, Golob J, Kelleher E, Ye Z, Pardoll D, Cheng L. Blood. 2002 Jan 15. 99(2):399-408. 10.1182/blood.V99.2.399 PubMed 11781219