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DR.GFP
(Plasmid #17617)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 17617 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    SIN LV pRLLhPGK.GFP Sin-18 (modified)
  • Backbone manufacturer
    Dr. Didier Trono
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    HLA-DRa promoter
  • Alt name
    (MHC II) HLA-DR alpha promoter
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    300
  • GenBank ID
    X00274
  • Entrez Gene
    HLA-DRA (a.k.a. HLA-DRA1)
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

Resource Information

Depositor Comments

SIN lentiviral vector that expresses GFP controlled by the promoter of the human HLA-DRalpha gene, which is selectively expressed in antigen-presenting cells (APCs).

We constructed DR.GFP by using the human (MHC II) HLA-DRalpha promoter (nucleotide 182 to 480, as numbered in GenBank accession X00274) to replace the PGK promoter (XhoI to BamHI sites in the PGK.GFP vector). This 300-base pair promoter sequence has been shown to confer selective expression of a transgene in MHC II+ cells.

Recombinant LVs were generated using the 3-plasmid system by cotransfection of 293T cells through calcium phosphate precipitation. The ratio of a transducing vector, pMD.G, and pCMVDeltaR8.91 was fixed at 1.5:0.5:2 µg for 10^6 293T cells.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DR.GFP was a gift from Linzhao Cheng (Addgene plasmid # 17617)
  • For your References section:

    Targeting transgene expression to antigen-presenting cells derived from lentivirus-transduced engrafting human hematopoietic stem/progenitor cells. Cui Y, Golob J, Kelleher E, Ye Z, Pardoll D, Cheng L. Blood. 2002 Jan 15. 99(2):399-408. 10.1182/blood.V99.2.399 PubMed 11781219