Addgene - pCMV-Tag1-NS5A Plasmid Data

Plasmid 17646: pCMV-Tag1-NS5A

Gene/insert name:
HCV NS5A
Alt name:
NS5A
Alt name:
HCV non-structural (NS) protein 5A
Insert size:
1400
Species:
Hepatitis C virus
Entrez Gene:
HCVgp1 (HCVgp1)
Fusion protein or tag:
Flag
Terminal:
N terminal on backbone
Vector backbone:
pCMVTag1
(Search Vector Database)
Backbone manufacturer:
Stratagene
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4300
Cloning site 5':
BglII
Site destroyed during cloning:
No
Cloning site 3':
KpnI
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (2)
Principal Investigator:
Xin Wang
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Induction of a unique gene expression profile in primary human hepatocytes by hepatitis C virus core, NS3 and NS5A proteins. Budhu et al (Carcinogenesis. 2007 Jul . 28(7):1552-60. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17646" in your Materials and Methods section.