Piggybac-TetO-hNurr1-blast
(Plasmid #176485)

• Purpose: TetO-hNurr1 in a PiggyBac vector with Blasticidin resistance
• Depositing Lab: Marius Wernig
• Publication: Ng et al Stem Cell Reports. 2021 Jul 13;16(7):1763-1776. doi: 10.1016/j.stemcr.2021.05.017. Epub 2021 Jun 24.

Backbone

• Vector backbone: Piggybac TetO - Blastocidin
• Backbone size w/o insert (bp): 11000
• Total vector size (bp): 9000
• Modifications to backbone: estimated sizes
• Vector type: Mammalian Expression
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: The Wernig lab uses Stbl3 or JM109 for downstream applications.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Nurr1
• Species: H. sapiens (human)
• Insert Size (bp): 1797
• Entrez Gene: NR4A2 (a.k.a. HZF-3, IDLDP, NOT, NURR1, RNR1, TINUR)
• Promoter: TetO

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Mfe1 (unknown if destroyed)
• 3′ cloning site: PacI (unknown if destroyed)
• 5′ sequencing primer: CCTTGTGTTCAGGCGCAG
• 3′ sequencing primer: TTAGAAAGGTAAGGTGTC

Resource Information

• Supplemental Documents:
  • Piggybac-TetO-hNurr1-blast (hNurr1 sequence only).gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry