Addgene - pYESir2HY-puro Plasmid Data

Plasmid 1770: pYESir2HY-puro

Gene/insert name:
Sir2
Alt name:
silent information regulator
Insert size:
2500
Species:
H. sapiens (human)
Entrez Gene:
SIRT1 (RP11-57G10.3, SIR2L1)
Mutation:
H363Y, residue 363 changed from histidine to tyrosine, dominant negative
Vector backbone:
pBABE-puro
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
5169
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
SnaBI
Site destroyed during cloning:
No
5' sequencing primer:
pBABE 5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Comments:

SspI site in Amp gene.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: hSIR2(SIRT1) functions as an NAD-dependent p53 deacetylase. Vaziri et al (Cell 2001 Oct 19;107(2):149-59. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1770" in your Materials and Methods section.