Addgene - pCLEN Plasmid Data

Plasmid 17704: pCLEN

Gene/insert name:
NIC
Alt name:
Notch intracellular domain
Alt name:
Notch
Insert size:
1380
Species:
M. musculus (mouse)
Entrez Gene:
Notch1 (RP23-306D20.12-001, 9930111A19Rik, Mis6, N1, Tan1, lin-12)
Fusion protein or tag:
Myc
Terminal:
N terminal on insert
Vector backbone:
pCLE
(Search Vector Database)
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
7600
5' sequencing primer:
na List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Nicholas Gaiano
Terms and Licenses:
MTA

Comments:

The portion of mouse Notch1 (NIC) used spans residues 1753–2185, and has a Myc tag at the N terminus.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Radial glial identity is promoted by Notch1 signaling in the murine forebrain. Gaiano et al (Neuron. 2000 May . 26(2):395-404. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 17704" in your Materials and Methods section.