|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||1779||Plasmid sent as bacteria in agar stab||1||$65||Add to Cart|
- Backbone size w/o insert (bp) 4800
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namelarge T antigen
Alt namelarge T cDNA
Insert Size (bp)2100
Entrez GeneSV40gp6 (a.k.a. SV40gp6)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer pBABE 5' (Common Sequencing Primers)
SspI site in Amp gene.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBABE-zeo largeTcDNA was a gift from Bob Weinberg (Addgene plasmid # 1779)
For your References section:Enumeration of the simian virus 40 early region elements necessary for human cell transformation. Hahn WC, Dessain SK, Brooks MW, King JE, Elenbaas B, Sabatini DM, DeCaprio JA, Weinberg RA. Mol Cell Biol 2002 Apr;22(7):2111-23. 10.1128/MCB.22.7.2111-2123.2002 PubMed 11884599
Generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.