HI.HslU(delI)V.RSET.wt(Nde1).107-GG-244
(Plasmid #17859)

• Depositing Lab: David McKay
• Publication: Kwon et al J Mol Biol. 2003 Jul 4. 330(2):185-95.

Backbone

• Vector backbone: pRSET_B
• Backbone size w/o insert (bp): 2800
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: BL21(DE3)
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Haemophilus influenzae heat shock loci U and V
• Alt name: H. influenzae HslUV
• Species: Haemophilus influenzae
• Insert Size (bp): 1470
• Mutation: coding sequence for residues 108-243 of HslU (the "I domains") replaced with coding sequence for two glycines

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (unknown if destroyed)
• 3′ cloning site: KpnI (unknown if destroyed)
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Insert for full-length HslUV cloned from two plasmids of the H. influenzae genomic sequencing project obtained from ATCC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Also used to express and purify HslV protein alone, which is expressed in excess over HslU.