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FLAG-mBAF45b IRES GFP
(Plasmid #17881)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    Beta-actin/CMV-IRES-EGFP
  • Backbone size w/o insert (bp) 6000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    BAF45b
  • Alt name
    Neud4
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1166
  • Entrez Gene
    Dpf1 (a.k.a. Neud4)
  • Tags / Fusion Proteins
    • FLAG (N terminal on insert)
    • IRES EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (destroyed during cloning)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer IRES-R
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLAG-mBAF45b IRES GFP was a gift from Jerry Crabtree (Addgene plasmid # 17881)
  • For your References section:

    An essential switch in subunit composition of a chromatin remodeling complex during neural development. Lessard J, Wu JI, Ranish JA, Wan M, Winslow MM, Staahl BT, Wu H, Aebersold R, Graef IA, Crabtree GR. Neuron. 2007 Jul 19. 55(2):201-15. 10.1016/j.neuron.2007.06.019 PubMed 17640523