Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17920||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepLKO.1 puro
- Backbone size w/o insert (bp) 7200
Vector typeMammalian Expression, Lentiviral, RNAi
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)50
- Cloning method Restriction Enzyme
- 5′ cloning site Age1 (not destroyed)
- 3′ cloning site EcoR1 (not destroyed)
- 5′ sequencing primer NA (Common Sequencing Primers)
Scrambled hairpin (negative control, shSCR)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLKO.1 shSCR was a gift from Sheila Stewart (Addgene plasmid # 17920 ; http://n2t.net/addgene:17920 ; RRID:Addgene_17920)
For your References section:Flap endonuclease 1 contributes to telomere stability. Saharia A, Guittat L, Crocker S, Lim A, Steffen M, Kulkarni S, Stewart SA. Curr Biol. 2008 Apr 8. 18(7):496-500. 10.1016/j.cub.2008.02.071 PubMed 18394896