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pTEK-4S-0X
(Plasmid #17974)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 17974 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMS2
  • Vector type
    mycobacteria expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    This plasmid is high copy number in E. coli and low copy number in mycobacteria.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tetR(B)r1.7
  • Alt name
    reverse tetR

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

revTetR is expressed from a strong mycobacterial promoter (psmyc = puv15). Also see Pubmed ID 18059281.

Kanamycin resistant derivative of pTE-4S0X.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTEK-4S-0X was a gift from Sabine Ehrt (Addgene plasmid # 17974 ; http://n2t.net/addgene:17974 ; RRID:Addgene_17974)
  • For your References section:

    Silencing Mycobacterium smegmatis by using tetracycline repressors. Guo XV, Monteleone M, Klotzsche M, Kamionka A, Hillen W, Braunstein M, Ehrt S, Schnappinger D. J Bacteriol. 2007 Jul . 189(13):4614-23. 10.1128/JB.00216-07 PubMed 17483222