pNIC28-Bsa4_ZV-NS5-RdRp
(Plasmid
#179847)
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PurposeThe RdRp domain (with a 6-histidine tag) from Zika virus nonstructural protein 5 (French Polynesia strain, GenBank accession number ARU07075.1) encompassing residues 270 to 89
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 179847 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepNIC-Bsa4
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Backbone manufacturerBiobasics, Singapore
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameZika Virus Non-structural protein 5 RdRp domain
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Alt nameZikV NS5 RdRp
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Tag
/ Fusion Protein
- 6-histine
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNIC28-Bsa4_ZV-NS5-RdRp was a gift from Julien Lescar (Addgene plasmid # 179847 ; http://n2t.net/addgene:179847 ; RRID:Addgene_179847) -
For your References section:
Non-nucleoside Inhibitors of Zika Virus RNA-Dependent RNA Polymerase. Gharbi-Ayachi A, Santhanakrishnan S, Wong YH, Chan KWK, Tan ST, Bates RW, Vasudevan SG, El Sahili A, Lescar J. J Virol. 2020 Oct 14;94(21). pii: JVI.00794-20. doi: 10.1128/JVI.00794-20. Print 2020 Oct 14. 10.1128/JVI.00794-20 PubMed 32796069
