Addgene - pB1H2w2-12-Oct1 Plasmid Data

Plasmid 18041: pB1H2w2-12-Oct1

Gene/insert name:
HD of Oct1
Insert size:
206
Species:
mutant
Mutation:
the homeodomain of Oct1 is fused to fingers 1 and 2 of zif268 (previously described by the Pabo lab, ZFHD1). To be used as a positive control.
Vector backbone:
ColE1
(Search Vector Database)
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
3878
Cloning site 5':
Kpn1
Site destroyed during cloning:
No
Cloning site 3':
Xba1
Site destroyed during cloning:
No
5' sequencing primer:
OMG5 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Principal Investigator:
Scot Wolfe
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A systematic characterization of factors that regulate Drosophila segmentation via a bacterial one-hybrid system. Noyes et al (Nucleic Acids Res. 2008 Mar 10. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 18041" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

12609: pH3U3-mcs

18039: pB1H2w5-Prd

18045: pB1H2w2-zif268

18049: USO hisB- pyrF- rpoZ-

18043: pB1H2w5-12-C81S