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Plasmid 18043: pB1H2w5-12-C81S
  • HhaI C81S mutant

  • 1031

  • mutant

  • Simply used as an insert between Kpn1 and Xba1

  • ColE1
    (Search Vector Database)

  • Bacterial Expression

  • 3878

  • Kpn1

  • No

  • Xba1

  • No

  • OMG5 List of Sequencing Primers

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • View sequences (3)
  • Scot Wolfe

  • UBMTA

Comments: 

There is a 2bp difference in the promoter region between this construct and Addgene plasmid 18042.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: A systematic characterization of factors that regulate Drosophila segmentation via a bacterial one-hybrid system. Noyes et al (Nucleic Acids Res. 2008 Mar 10. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 18043" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only