pRQ-hRasV12
(Plasmid #18113)

• Depositing Lab: Alex Swarbrick
• Publication: Swarbrick et al Proc Natl Acad Sci U S A. 2008 Apr 8. 105(14):5402-7.

Backbone

• Vector backbone: N/A
• Backbone manufacturer: Constructed from multiple sources
• Backbone size w/o insert (bp): 6664
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl3
• Growth instructions: STBL2 30'C
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: h-RasV12G
• Alt name: harvey Ras
• Alt name: oncogenic ras
• Species: H. sapiens (human)
• Insert Size (bp): 569
• Mutation: G12V, constitutively active
• GenBank ID: NM_005343
• Entrez Gene: HRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ cloning site: Gateway (unknown if destroyed)
• 3′ cloning site: gateway (unknown if destroyed)
• 5′ sequencing primer: GCT CGT TTA GTG AAC CGT CA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry