pEH_AAV_TERT_prom_-146C>T_C7C_1.8kb
(Plasmid #183081)

• Purpose: rAAV transfer plasmid with ITRs flanking: (1) TERT -146C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.
• Depositing Lab: Aviv Regev
• Publication: Hodis et al Science. 2022 Apr 29;376(6592):eabi8175. doi: 10.1126/science.abi8175. Epub 2022 Apr 29.

Backbone

• Vector backbone: AAV
• Backbone manufacturer: Received from R. Platt from F. Zhang lab, Broad Institute
• Backbone size w/o insert (bp): 4153
• Total vector size (bp): 5993
• Modifications to backbone: Replaced all sequence between AAV ITRs with insert acceptance site and PGK-Puro-PolyA.
• Vector type: AAV ; Homologous Recombination Donor Template
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TERT -146C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms
• Alt name: TERT promoter and exon 1
• Species: H. sapiens (human)
• Insert Size (bp): 1840
• Mutation: -146C>T promoter, Exon 1 C7C (C21>T21) silent mutation
• Entrez Gene: TERT (a.k.a. CMM9, DKCA2, DKCB4, EST2, PFBMFT1, TCS1, TP2, TRT, hEST2, hTRT)
• Promoter: None for primary insert (Though partial TERT promoter is part of donor template). PGK for puromycin resistance gene.

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: MluI (not destroyed)
• 3′ cloning site: AgeI (not destroyed)
• 5′ sequencing primer: NGS
• 3′ sequencing primer: NGS

Resource Information

• Supplemental Documents:
  • peh_aav_tert_prom_-146ct_c7c_1-8kb.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry