pRET.IL (No IRES-EGFP)
(Plasmid #1832)

• Purpose: (Empty Backbone)
• Depositing Lab: Philip Leder
• Publication: Ishida et al Nucleic Acids Res 1999 Dec 15;27(24):e35.

Backbone

• Vector backbone: pRET
• Vector type: Mammalian Expression, Retroviral, Cre/Lox
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Alt name: retroviral RET construct
• Alt name: removable exon trap
• Alt name: Long (strong) RNA pol II promoter for NEO expression

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: na

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See scanned map.
This is the original RET construct for in vitro mutagenesis experiments. The highest virus titer that can be obtained with this construct is ~4x10^4 cfu/ml: the mRNA instability signal seems to block transcription of the virus genome at least to some extent. This construct can not be used for expresssion pattern analysis because of lack of IRES-EGFP. The XbaI-XbaI fragment can be used for transfection. Enhancer sequence in the U3 portion of the 5' LTR is not included in this XbaI-XbaI fragment.
(Leder #C-1021)