Plasmid 1832: pRET.IL (No IRES-EGFP)

• Gene/insert name: None
• Alt name: retroviral RET construct
• Alt name: removable exon trap
• Alt name: Long (strong) RNA pol II promoter for NEO expression
• Vector backbone: pRET
  (Search Vector Database)
• Vector type: Mammalian Expression, Retroviral, Cre/Lox
• 5' sequencing primer: na
• Bacterial resistance(s) Ampicillin
• Growth strain(s) DH5alpha
• Growth temperature (℃): 37
• High or low copy: High Copy
• Selectable markers: Neomycin
• Sequence: View sequences (1)
• Map: View map
• Principal Investigator: Philip Leder
• Terms and Licenses: MTA

Comments: 

See scanned map.
This is the original RET construct for in vitro mutagenesis experiments. The highest virus titer that can be obtained with this construct is ~4x10^4 cfu/ml: the mRNA instability signal seems to block transcription of the virus genome at least to some extent. This construct can not be used for expresssion pattern analysis because of lack of IRES-EGFP. The XbaI-XbaI fragment can be used for transfection. Enhancer sequence in the U3 portion of the 5' LTR is not included in this XbaI-XbaI fragment.
(Leder #C-1021)

Article: RET: a poly A-trap retrovirus vector for reversible disruption and expression monitoring of genes in living cells. Ishida et al (Nucleic Acids Res 1999 Dec 15;27(24):e35. PubMed)