VPS4B, S2C
(Plasmid
#184806)
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PurposeBacterial expression of VPS4B (S2C mutation)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 184806 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbone2BT
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameVPS4B
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SpeciesH. sapiens (human)
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MutationS2C
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Entrez GeneVPS4B (a.k.a. MIG1, SKD1, SKD1B, VPS4-2)
- Promoter T7
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Tag
/ Fusion Protein
- His6 (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
6His-TEVsite-VPS4B-S2C-stop.
Please visit https://www.biorxiv.org/content/10.1101/2022.02.03.479062v2 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
VPS4B, S2C was a gift from James Hurley (Addgene plasmid # 184806 ; http://n2t.net/addgene:184806 ; RRID:Addgene_184806) -
For your References section:
Friction-driven membrane scission by the human ESCRT-III proteins CHMP1B and IST1. Cada AK, Pavlin MR, Castillo JP, Tong AB, Larsen KP, Ren X, Yokom AL, Tsai FC, Shiah JV, Bassereau PM, Bustamante CJ, Hurley JH. Proc Natl Acad Sci U S A. 2022 Jul 19;119(29):e2204536119. doi: 10.1073/pnas.2204536119. Epub 2022 Jul 11. 10.1073/pnas.2204536119 PubMed 35858336
