Addgene - myc-Raptor Plasmid Data

Plasmid 1859: myc-Raptor

Gene/insert name:
Raptor
Insert size:
6000
Species:
H. sapiens (human)
Entrez Gene:
RPTOR (KOG1, Mip1)
Fusion protein or tag:
Myc
Terminal:
N terminal on backbone
Vector backbone:
pRK-5
(Search Vector Database)
Backbone manufacturer:
BD Pharmingen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4754
Cloning site 5':
SalI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
SP6 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Map:
View map
Supplemental document:
Notes from Addgene (1)
Principal Investigator:
David Sabatini
Terms and Licenses:
MTA

Comments:

Addgene has compiled full sequence for this plasmid. Please see Notes link.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Rictor, a novel binding partner of mTOR, defines a rapamycin-insensitive and raptor-independent pathway that regulates the cytoskeleton. Sarbassov et al (Curr Biol 2004 Jul 27;14(14):1296-302. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1859" in your Materials and Methods section.