Addgene - pRS416 Gal-RNQ1 Plasmid Data

Plasmid 18684: pRS416 Gal-RNQ1

Gene/insert name:
Rnq1
Alt name:
Gal-Rnq1 p416
Alt name:
Gal Rnq1
Insert size:
1218
Species:
S. cerevisiae (budding yeast)
Entrez Gene:
RNQ1 (YCL028W)
Vector backbone:
pRS416 Gal
(Search Vector Database)
Vector type:
Yeast Expression
Backbone size w/o insert (bp):
5574
Cloning site 5':
Xba1
Site destroyed during cloning:
No
Cloning site 3':
BamH1
Site destroyed during cloning:
No
5' sequencing primer:
From Gal Promoter GTTAATATACCTCTATACTTTAACGTCAAGGAGA List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Low Copy
Selectable markers:
URA3
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Susan Lindquist
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Chaperone-dependent amyloid assembly protects cells from prion toxicity. Douglas et al (Proc Natl Acad Sci U S A. 2008 May 20. 105(20):7206-11. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 18684" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

18685: pRS416 Gal-RNQ1-YFP