|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||18693||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4000
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)600
MutationA206K mutation in paGFP
Entrez GeneHRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras)
/ Fusion Protein
- photoactivatable GFP (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7 (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byM. Matsuda (H-Ras) J. Lippincott-Schwartz (paGFP)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:paGFP-HRas was a gift from Karel Svoboda (Addgene plasmid # 18693)
Generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.