paGFP-HRas
(Plasmid #18693)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pCI
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    H-Ras
  • Alt name
    HRas
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    600
  • Mutation
    A206K mutation in paGFP
  • Entrez Gene
    HRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras)
  • Tag / Fusion Protein
    • photoactivatable GFP (N terminal on insert)

Cloning Information

Resource Information

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    paGFP-HRas was a gift from Karel Svoboda (Addgene plasmid # 18693)