p-transposase
(Plasmid #18699)

• Depositing Lab: Nancy Craig
• Publication: Mitra et al EMBO J. 2008 Apr 9. 27(7):1097-109.

Backbone

• Vector backbone: pBAD/Myc-HisC
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4093
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: piggyBac transposase
• Alt name: T.ni piggyBac transposase ORF
• Species: Trichoplusia ni
• Insert Size (bp): 1782
• Mutation: N/A
• GenBank ID: DQ340395
• Tags / Fusion Proteins:
  • Myc (C terminal on backbone)
  • His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: gattagcggatcctacctg
• 3′ sequencing primer: gacggcgctattcagatc

Resource Information

• A portion of this plasmid was derived from a plasmid made by: David A. O'Brochta, Ph.D Professor Center for Biosystems Research University of Maryland Biotechnology Institute Room 5141 Plant Sciences Building College Park, MD 20742 Phone: 301-405-7680 Fax: 301-314-9075 Email :

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry