Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pCMV/myc/Nuc-hOGG1
(Plasmid #18709)

Loading...

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 18709 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMCV/myc/nuc
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    8-oxoguanine DNA glycosylase
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1035
  • Mutation
    N/A
  • GenBank ID
    NM_002542
  • Entrez Gene
    OGG1 (a.k.a. HMMH, HOGG1, MUTM, OGH1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer cgcaaatgggcggtag
  • 3′ sequencing primer BGH reverse
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV/myc/Nuc-hOGG1 was a gift from David Sidransky (Addgene plasmid # 18709 ; http://n2t.net/addgene:18709 ; RRID:Addgene_18709)
  • For your References section:

    Targeting of mutant hogg1 in mammalian mitochondria and nucleus: effect on cellular survival upon oxidative stress. Chatterjee A, Mambo E, Zhang Y, Deweese T, Sidransky D. BMC Cancer. 2006 . 6():235. 10.1186/1471-2407-6-235 PubMed 17018150