Addgene - pMSCV-FlagBcl10 Plasmid Data

Plasmid 18718: pMSCV-FlagBcl10

Gene/insert name:
B cell lymphoma 10
Alt name:
Bcl10
Insert size:
750
Species:
H. sapiens (human)
GenBank ID:
NM_003921
Entrez Gene:
BCL10 (RP11-234D19.2, CARMEN, CIPER, CLAP, c-E10, mE10)
Fusion protein or tag:
Flag
Terminal:
C terminal on insert
Vector backbone:
pMSCVpuro
(Search Vector Database)
Backbone manufacturer:
BD Clontech
Vector type:
Retroviral
Backbone size w/o insert (bp):
6300
Cloning site 5':
Xho I
Site destroyed during cloning:
No
Cloning site 3':
EcoR I
Site destroyed during cloning:
No
5' sequencing primer:
pMSCV primer List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (1)
Principal Investigator:
Jon Ashwell
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: NEMO recognition of ubiquitinated Bcl10 is required for T cell receptor-mediated NF-kappaB activation. Wu et al (Proc Natl Acad Sci U S A. 2008 Feb 26. 105(8):3023-8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 18718" in your Materials and Methods section.