Addgene - pBabe H-Ras V12 S35 Plasmid Data

Plasmid 18746: pBabe H-Ras V12 S35

Gene/insert name:
H-Ras V12 S35
Alt name:
HRas V12 S35
Alt name:
H-Ras
Insert size:
700
Species:
H. sapiens (human)
GenBank ID:
NM_005343
Entrez Gene:
HRAS (C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, K-RAS, N-RAS, RASH1)
Mutation:
V12 S35
Vector backbone:
pBabe-puro
(Search Vector Database)
Backbone manufacturer:
Available at Addgene (#1764)
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
5100
Cloning site 5':
XhoI / SalI
Site destroyed during cloning:
Yes
Cloning site 3':
BglII / BamHI
Site destroyed during cloning:
Yes
5' sequencing primer:
pBabe 5' List of Sequencing Primers
3' sequencing primer:
pBabe 3'
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Person or lab that originally
cloned the gene/insert:
Jay Morgenstern
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Scott Lowe
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 18746" in your Materials and Methods section.