Addgene - 1352wUV2 F1bbs kan GCGTGGNNN Plasmid Data

Plasmid 18753: 1352wUV2 F1bbs kan GCGTGGNNN

Gene/insert name:
3 tandem zinc fingers
Insert size:
1298
Species:
M. musculus (mouse)
Mutation:
A Kan cassette (1090bp) is inserted between two bbsI sites in finger 1 of this construct. This cassette is here to provide a large drop out band when digested with BbsI. The digested backbone can then be used for building finger 1 libraries. Fingers two and three are anchor fingers which specify GCGTGG.
Vector backbone:
ColE1
(Search Vector Database)
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
3890
Cloning site 5':
BbsI
Site destroyed during cloning:
No
Cloning site 3':
BbsI
Site destroyed during cloning:
No
5' sequencing primer:
OMG5 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin and Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Scot Wolfe
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Targeted gene inactivation in zebrafish using engineered zinc-finger nucleases. Meng et al (Nat Biotechnol. 2008 Jun . 26(6):695-701. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 18753" in your Materials and Methods section.