A Kan cassette (1090bp) is inserted between two bbsI sites in finger 3 of this construct. This cassette is here to provide a large drop out band when digested with BbsI. The digested backbone can then be used for building finger 3 libraries. Fingers one and two are anchor fingers which specify TGGGCGk.
Please acknowledge the principal investigator and cite this article if you use
this plasmid in a publication. Also, please include the text "Addgene plasmid
18756" in your Materials and Methods section.