1
Plasmid 18756: 1352wUV2 F3bbs kan NNNTGGGCGk
  • 3 tandem zinc fingers

  • 1298

  • M. musculus (mouse)

  • A Kan cassette (1090bp) is inserted between two bbsI sites in finger 3 of this construct. This cassette is here to provide a large drop out band when digested with BbsI. The digested backbone can then be used for building finger 3 libraries. Fingers one and two are anchor fingers which specify TGGGCGk.

  • ColE1
    (Search Vector Database)

  • Bacterial Expression

  • 3890

  • BbsI

  • No

  • BbsI

  • No

  • OMG5 List of Sequencing Primers

  • Ampicillin and Kanamycin

  • DH5alpha

  • 37

  • High Copy

  • View sequences (2)
  • Scot Wolfe

  • UBMTA

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Targeted gene inactivation in zebrafish using engineered zinc-finger nucleases. Meng et al (Nat Biotechnol. 2008 Jun . 26(6):695-701. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 18756" in your Materials and Methods section.