1352wUV2 F3bbs kan NNNTGGGCGk
(Plasmid #18756)

Available to Academic and Nonprofits Only

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    3 tandem zinc fingers
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1298
  • Mutation
    A Kan cassette (1090bp) is inserted between two bbsI sites in finger 3 of this construct. This cassette is here to provide a large drop out band when digested with BbsI. The digested backbone can then be used for building finger 3 libraries. Fingers one and two are anchor fingers which specify TGGGCGk.

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BbsI (not destroyed)
  • 3′ cloning site BbsI (not destroyed)
  • 5′ sequencing primer OMG5
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    1352wUV2 F3bbs kan NNNTGGGCGk was a gift from Scot Wolfe (Addgene plasmid # 18756)
  • For your References section:

    Targeted gene inactivation in zebrafish using engineered zinc-finger nucleases. Meng X, Noyes MB, Zhu LJ, Lawson ND, Wolfe SA. Nat Biotechnol. 2008 Jun . 26(6):695-701. 10.1038/nbt1398 PubMed 18500337