Addgene - MSCV HAM Myc puro Plasmid Data

Plasmid 18775: MSCV HAM Myc puro

Gene/insert name:
myc
Insert size:
1461
Species:
H. sapiens (human)
Entrez Gene:
MYC (MRTL, bHLHe39, c-Myc)
Fusion protein or tag:
HA
Terminal:
N terminal on insert
Vector backbone:
pMSCVpuro
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
6300
Cloning site 5':
BglII
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
MSCV List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Scott Lowe
Terms and Licenses:
MTA

Comments:

HAM (a methionine-rich variant of the widely used haemagglutinin tag)-tagged myc in pMSCV puro.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Evasion of the p53 tumour surveillance network by tumour-derived MYC mutants. Hemann et al (Nature. 2005 Aug 11. 436(7052):807-11. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 18775" in your Materials and Methods section.