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pFLIPmal-40uDelta1YFP
(Plasmid #18853)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 18853 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRSET
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    optimized maltose FRET sensor
  • Species
    E. coli
  • Mutation
    N227A;
  • Tags / Fusion Proteins
    • CFP (N terminal on insert)
    • YFP (C terminal on insert)

Cloning Information

Resource Information

Depositor Comments

W230A mutation, which we introduced to change the affinity.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFLIPmal-40uDelta1YFP was a gift from Wolf Frommer (Addgene plasmid # 18853 ; http://n2t.net/addgene:18853 ; RRID:Addgene_18853)
  • For your References section:

    Fluorescence Resonance Energy Transfer sensors for quantitative monitoring of pentose and disaccharide accumulation in bacteria. Kaper T, Lager I, Looger LL, Chermak D, Frommer WB. Biotechnol Biofuels. 2008 Jun 3. 1(1):11. 10.1186/1754-6834-1-11 PubMed 18522753