pFLIPmal-40uDelta1Venus
(Plasmid #18854)

• Depositing Lab: Wolf Frommer
• Publication: Kaper et al Biotechnol Biofuels. 2008 Jun 3. 1(1):11.

Backbone

• Vector backbone: pRSET
• Backbone size w/o insert (bp): 4000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pFLIPmal-40uDelta1V
• Species: E. coli
• Mutation: N227A
• Tags / Fusion Proteins:
  • CFP (N terminal on backbone)
  • Venus (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

W230A mutation, which we introduced to change the affinity.