pDESTsmg
(Plasmid #18878)

• Purpose: (Empty Backbone)
• Depositing Lab: Ted Baker
• Publication: Goldstone et al Protein Expr Purif. 2008 Jan . 57(1):81-7.

Backbone

• Vector backbone: pYUB1049
• Backbone size (bp): 6573
• Vector type: Bacterial Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Growth instructions: This is a Gateway destination vector, so it requires a ccdB survival strain of E. coli for propagation of plasmid.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: None
• Tags / Fusion Proteins:
  • His (N terminal on backbone)
  • His (C terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: This plasmid was constructed by Rachael M. Goldstone from the pYUB1049 vector. pYUB1049 was donated by Mi-Sun Koo and William R. Jacobs from the Department of Microbiology and Immunology at the Albert Einstein College of Medicine, New York, USA.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is a Gateway destination vector. It requires a ccdB survival strain of E. coli for propagation of the plasmid, but once a gene of interest has been cloned in (via the LR reaction) it will grow in standard E. coli strains (eg. DH5alpha, BL21).

This is a shuttle vector, designed for expression of the inserted gene in Mycobacterium smegmatis as well as allowing cloning using E. coli.