pET16b-Mms2
(Plasmid #18893)

• Depositing Lab: Cecile Pickart
• Publication: Raasi et al Methods Mol Biol. 2005 . 301():47-55.

Backbone

• Vector backbone: pET16b
• Backbone size w/o insert (bp): 5700
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: BL21pJY2
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Yeast ubiquitin conjugating enzyme Mms2
• Species: S. cerevisiae (budding yeast)
• Entrez Gene: MMS2 (a.k.a. YGL087C)
• Tag / Fusion Protein:
  • HIS10 (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: This plasmid is constructed by Hofmann R.M.
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Hofmann R.M. Pickart C.M. 1999, Cell 96, 645-653