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pYBS139
(Plasmid #18900)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 18900 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pJG4-5
  • Backbone size w/o insert (bp) 6449
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    FAR1
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    6500
  • Entrez Gene
    FAR1 (a.k.a. YJL157C)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pYBS139 is a derivative of pYBS135 in which the EcoRI site was filled n to create the proper reading frame.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYBS139 was a gift from Elaine Elion (Addgene plasmid # 18900 ; http://n2t.net/addgene:18900 ; RRID:Addgene_18900)
  • For your References section:

    The MAP kinase Fus3 associates with and phosphorylates the upstream signaling component Ste5. Kranz JE, Satterberg B, Elion EA. Genes Dev. 1994 Feb 1. 8(3):313-27. 10.1101/gad.8.3.313 PubMed 8314085